Pathogenic and pandemic Vibrio parahaemolyticus detection in fish and shellfish isolates
نویسندگان
چکیده
Anjay, S.C. Das, A. Kumar, P. Kaushik & Savita Kumari Eastern Regional Station, IVRI, Belgachia, Kolkata-700 037, India. Department of Veterinary Public Health, Bihar Veterinary College, Patna800 014, Bihar, India Division of Veterinary Public Health, E.R.S., I.V.R.I., Belgachia, Kolkata700 037, West Bengal, India Division of Veterinary Public Health, I.V.R.I., Izatnagar, Bareilly243 122, Uttar Pradesh, India Department of Veterinary Microbiology, B.V.C., Patna800 014, Bihar, India *[E-mail: [email protected] ]
منابع مشابه
A PCR Assay for Specific Detection of the Pandemic Vibrio parahaemolyticus O3:K6 Clone from Shellfish
The current standard method for identifying Vibrio parahaemolyticus serotype O3:K6, an emerging pathogen with apparent enhanced virulence characteristics, typically takes 4 to 6 d to complete and requires serotyping. To provide a more rapid strategy, we optimized a polymerase chain reaction (PCR)-based assay for specific detection of V. parahaemolyticus O3:K6. Of 78 V. parahaemolyticus isolates...
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In spite of the potential risk involved with contamination of seafood with Vibrio parahaemolyticus, there is a lack of information on the occurrence of pathogenic V. parahaemolyticus in Europe. This organism was isolated in 1999 from a large outbreak (64 cases admitted to a single hospital) associated with raw oyster consumption in Galicia, Spain, one of the most important regions in shellfish ...
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Recent Vibrio parahaemolyticus outbreaks associated with consumption of raw shellfish in the United States focused attention on the occurrence of this organism in shellfish. From March 1999 through September 2000, paired oyster samples were collected biweekly from two shellfish-growing areas in Mobile Bay, Ala. The presence and densities of V. parahaemolyticus were determined by using DNA probe...
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UNLABELLED Vibrio parahaemolyticus is a marine bacterium which is also responsible for acute diarrhoeal illness in human beings. Eating raw seafish or contaminated seafood is responsible for acute gastroenteritis. The aim of this study was to investigate the isolation, identification and molecular characterization of Vibrio parahaemolyticus from the fish samples in Kolkata, India. MATERIALS A...
متن کاملDetection of Vibrio parahaemolyticus in shellfish by use of multiplexed real-time PCR with TaqMan fluorescent probes.
We developed a multiplexed real-time PCR assay using four sets of gene-specific oligonucleotide primers and four TaqMan probes labeled with four different fluorophores in a single reaction for detection of total and pathogenic Vibrio parahaemolyticus, including the pandemic O3:K6 serotype in oysters. V. parahaemolyticus has been associated with outbreaks of food-borne gastroenteritis caused by ...
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